bcl-2 (d17c4) Search Results


bcl2  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc bcl2
(A) The chemical structure of Mcl1-specific AUTAC consists of a MCL1 binding moiety connected to a FBnG-tag via a pyrazole linker. (B) Western blot analysis for Mcl1, <t>Bcl2,</t> Bclxl, and LC3I/II proteins in U266B1 cells treated with the indicated concentrations of AUTAC for 24h and 48h. GAPDH was used as the loading control. (C) Confocal images with their quantification for Mcl1 and LC3B proteins in U266B1 cells treated with AUTAC (5 μM) for 24 and 48h (scale 10 μM). (D) Western blot analysis for Mcl1, and LC3I/II proteins in U266B1 cells treated with different combinations of AUTAC (5 μM), CQ (20 μM) or 3-MA (1 mM) for 24h. GAPDH was used as the loading control. (E) Western blot analysis for ATG5, Mcl1, and LC3I/II proteins in U266B1 wild-type and U266B1 ATG5 KD cells treated with AUTAC (5 μM) for 24h. GAPDH was used as the loading control. (F) The percentage of cellular viability for U266B1 cells treated with the indicated concentrations AUTAC for 24, 48 and 72h. (G) Viability of AC16 cardiomyocytes treated with the indicated concentrations of AUTAC for 24, 48 and 72h. Three biological replicates for each cell line were used to perform cell viability assay and Western blotting. * p < 0. 05, ** p < 0.01, and **** p < 0.0001 indicates statistical significance of each condition compared to the indicated control as determined using two-way ANOVA with Sidak’s post hoc test.
Bcl2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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resource source identifier anti bax cst  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc resource source identifier anti bax cst
(A) The chemical structure of Mcl1-specific AUTAC consists of a MCL1 binding moiety connected to a FBnG-tag via a pyrazole linker. (B) Western blot analysis for Mcl1, <t>Bcl2,</t> Bclxl, and LC3I/II proteins in U266B1 cells treated with the indicated concentrations of AUTAC for 24h and 48h. GAPDH was used as the loading control. (C) Confocal images with their quantification for Mcl1 and LC3B proteins in U266B1 cells treated with AUTAC (5 μM) for 24 and 48h (scale 10 μM). (D) Western blot analysis for Mcl1, and LC3I/II proteins in U266B1 cells treated with different combinations of AUTAC (5 μM), CQ (20 μM) or 3-MA (1 mM) for 24h. GAPDH was used as the loading control. (E) Western blot analysis for ATG5, Mcl1, and LC3I/II proteins in U266B1 wild-type and U266B1 ATG5 KD cells treated with AUTAC (5 μM) for 24h. GAPDH was used as the loading control. (F) The percentage of cellular viability for U266B1 cells treated with the indicated concentrations AUTAC for 24, 48 and 72h. (G) Viability of AC16 cardiomyocytes treated with the indicated concentrations of AUTAC for 24, 48 and 72h. Three biological replicates for each cell line were used to perform cell viability assay and Western blotting. * p < 0. 05, ** p < 0.01, and **** p < 0.0001 indicates statistical significance of each condition compared to the indicated control as determined using two-way ANOVA with Sidak’s post hoc test.
Resource Source Identifier Anti Bax Cst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
resource source identifier anti bax cst - by Bioz Stars, 2026-09
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bcl 2  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology bcl 2
(A) The chemical structure of Mcl1-specific AUTAC consists of a MCL1 binding moiety connected to a FBnG-tag via a pyrazole linker. (B) Western blot analysis for Mcl1, <t>Bcl2,</t> Bclxl, and LC3I/II proteins in U266B1 cells treated with the indicated concentrations of AUTAC for 24h and 48h. GAPDH was used as the loading control. (C) Confocal images with their quantification for Mcl1 and LC3B proteins in U266B1 cells treated with AUTAC (5 μM) for 24 and 48h (scale 10 μM). (D) Western blot analysis for Mcl1, and LC3I/II proteins in U266B1 cells treated with different combinations of AUTAC (5 μM), CQ (20 μM) or 3-MA (1 mM) for 24h. GAPDH was used as the loading control. (E) Western blot analysis for ATG5, Mcl1, and LC3I/II proteins in U266B1 wild-type and U266B1 ATG5 KD cells treated with AUTAC (5 μM) for 24h. GAPDH was used as the loading control. (F) The percentage of cellular viability for U266B1 cells treated with the indicated concentrations AUTAC for 24, 48 and 72h. (G) Viability of AC16 cardiomyocytes treated with the indicated concentrations of AUTAC for 24, 48 and 72h. Three biological replicates for each cell line were used to perform cell viability assay and Western blotting. * p < 0. 05, ** p < 0.01, and **** p < 0.0001 indicates statistical significance of each condition compared to the indicated control as determined using two-way ANOVA with Sidak’s post hoc test.
Bcl 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl-2+(d17c4)/Bcl-2/pm35800619-82-22-43
Average 96 stars, based on 1 article reviews
bcl 2 - by Bioz Stars, 2026-09
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bcl 2  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc bcl 2
(A) The chemical structure of Mcl1-specific AUTAC consists of a MCL1 binding moiety connected to a FBnG-tag via a pyrazole linker. (B) Western blot analysis for Mcl1, <t>Bcl2,</t> Bclxl, and LC3I/II proteins in U266B1 cells treated with the indicated concentrations of AUTAC for 24h and 48h. GAPDH was used as the loading control. (C) Confocal images with their quantification for Mcl1 and LC3B proteins in U266B1 cells treated with AUTAC (5 μM) for 24 and 48h (scale 10 μM). (D) Western blot analysis for Mcl1, and LC3I/II proteins in U266B1 cells treated with different combinations of AUTAC (5 μM), CQ (20 μM) or 3-MA (1 mM) for 24h. GAPDH was used as the loading control. (E) Western blot analysis for ATG5, Mcl1, and LC3I/II proteins in U266B1 wild-type and U266B1 ATG5 KD cells treated with AUTAC (5 μM) for 24h. GAPDH was used as the loading control. (F) The percentage of cellular viability for U266B1 cells treated with the indicated concentrations AUTAC for 24, 48 and 72h. (G) Viability of AC16 cardiomyocytes treated with the indicated concentrations of AUTAC for 24, 48 and 72h. Three biological replicates for each cell line were used to perform cell viability assay and Western blotting. * p < 0. 05, ** p < 0.01, and **** p < 0.0001 indicates statistical significance of each condition compared to the indicated control as determined using two-way ANOVA with Sidak’s post hoc test.
Bcl 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl-2+(d17c4)/Bcl-2+Rabbit+mAb/10__1016_slash_j__ejmcr__2025__100316-179-5-8
Average 96 stars, based on 1 article reviews
bcl 2 - by Bioz Stars, 2026-09
96/100 stars
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bcl 2 d17c4  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc bcl 2 d17c4
(A) The chemical structure of Mcl1-specific AUTAC consists of a MCL1 binding moiety connected to a FBnG-tag via a pyrazole linker. (B) Western blot analysis for Mcl1, <t>Bcl2,</t> Bclxl, and LC3I/II proteins in U266B1 cells treated with the indicated concentrations of AUTAC for 24h and 48h. GAPDH was used as the loading control. (C) Confocal images with their quantification for Mcl1 and LC3B proteins in U266B1 cells treated with AUTAC (5 μM) for 24 and 48h (scale 10 μM). (D) Western blot analysis for Mcl1, and LC3I/II proteins in U266B1 cells treated with different combinations of AUTAC (5 μM), CQ (20 μM) or 3-MA (1 mM) for 24h. GAPDH was used as the loading control. (E) Western blot analysis for ATG5, Mcl1, and LC3I/II proteins in U266B1 wild-type and U266B1 ATG5 KD cells treated with AUTAC (5 μM) for 24h. GAPDH was used as the loading control. (F) The percentage of cellular viability for U266B1 cells treated with the indicated concentrations AUTAC for 24, 48 and 72h. (G) Viability of AC16 cardiomyocytes treated with the indicated concentrations of AUTAC for 24, 48 and 72h. Three biological replicates for each cell line were used to perform cell viability assay and Western blotting. * p < 0. 05, ** p < 0.01, and **** p < 0.0001 indicates statistical significance of each condition compared to the indicated control as determined using two-way ANOVA with Sidak’s post hoc test.
Bcl 2 D17c4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl-2+(d17c4)/Caspase-3+Rabbit+mAb/10__31857_slash_s0869813924090062-85-10-6
Average 97 stars, based on 1 article reviews
bcl 2 d17c4 - by Bioz Stars, 2026-09
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nrf2 d1z9c cell signaling  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc nrf2 d1z9c cell signaling
(A) The chemical structure of Mcl1-specific AUTAC consists of a MCL1 binding moiety connected to a FBnG-tag via a pyrazole linker. (B) Western blot analysis for Mcl1, <t>Bcl2,</t> Bclxl, and LC3I/II proteins in U266B1 cells treated with the indicated concentrations of AUTAC for 24h and 48h. GAPDH was used as the loading control. (C) Confocal images with their quantification for Mcl1 and LC3B proteins in U266B1 cells treated with AUTAC (5 μM) for 24 and 48h (scale 10 μM). (D) Western blot analysis for Mcl1, and LC3I/II proteins in U266B1 cells treated with different combinations of AUTAC (5 μM), CQ (20 μM) or 3-MA (1 mM) for 24h. GAPDH was used as the loading control. (E) Western blot analysis for ATG5, Mcl1, and LC3I/II proteins in U266B1 wild-type and U266B1 ATG5 KD cells treated with AUTAC (5 μM) for 24h. GAPDH was used as the loading control. (F) The percentage of cellular viability for U266B1 cells treated with the indicated concentrations AUTAC for 24, 48 and 72h. (G) Viability of AC16 cardiomyocytes treated with the indicated concentrations of AUTAC for 24, 48 and 72h. Three biological replicates for each cell line were used to perform cell viability assay and Western blotting. * p < 0. 05, ** p < 0.01, and **** p < 0.0001 indicates statistical significance of each condition compared to the indicated control as determined using two-way ANOVA with Sidak’s post hoc test.
Nrf2 D1z9c Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bcl 2 d17c4 antibody  (Proteintech)
96
Proteintech bcl 2 d17c4 antibody
(A) The chemical structure of Mcl1-specific AUTAC consists of a MCL1 binding moiety connected to a FBnG-tag via a pyrazole linker. (B) Western blot analysis for Mcl1, <t>Bcl2,</t> Bclxl, and LC3I/II proteins in U266B1 cells treated with the indicated concentrations of AUTAC for 24h and 48h. GAPDH was used as the loading control. (C) Confocal images with their quantification for Mcl1 and LC3B proteins in U266B1 cells treated with AUTAC (5 μM) for 24 and 48h (scale 10 μM). (D) Western blot analysis for Mcl1, and LC3I/II proteins in U266B1 cells treated with different combinations of AUTAC (5 μM), CQ (20 μM) or 3-MA (1 mM) for 24h. GAPDH was used as the loading control. (E) Western blot analysis for ATG5, Mcl1, and LC3I/II proteins in U266B1 wild-type and U266B1 ATG5 KD cells treated with AUTAC (5 μM) for 24h. GAPDH was used as the loading control. (F) The percentage of cellular viability for U266B1 cells treated with the indicated concentrations AUTAC for 24, 48 and 72h. (G) Viability of AC16 cardiomyocytes treated with the indicated concentrations of AUTAC for 24, 48 and 72h. Three biological replicates for each cell line were used to perform cell viability assay and Western blotting. * p < 0. 05, ** p < 0.01, and **** p < 0.0001 indicates statistical significance of each condition compared to the indicated control as determined using two-way ANOVA with Sidak’s post hoc test.
Bcl 2 D17c4 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl-2+(d17c4)/RIP3+Antibody/pmc11470026-400-89-114
Average 96 stars, based on 1 article reviews
bcl 2 d17c4 antibody - by Bioz Stars, 2026-09
96/100 stars
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rabbit cst bcl2 d17c4 rabbit mab  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc rabbit cst bcl2 d17c4 rabbit mab
(A) The chemical structure of Mcl1-specific AUTAC consists of a MCL1 binding moiety connected to a FBnG-tag via a pyrazole linker. (B) Western blot analysis for Mcl1, <t>Bcl2,</t> Bclxl, and LC3I/II proteins in U266B1 cells treated with the indicated concentrations of AUTAC for 24h and 48h. GAPDH was used as the loading control. (C) Confocal images with their quantification for Mcl1 and LC3B proteins in U266B1 cells treated with AUTAC (5 μM) for 24 and 48h (scale 10 μM). (D) Western blot analysis for Mcl1, and LC3I/II proteins in U266B1 cells treated with different combinations of AUTAC (5 μM), CQ (20 μM) or 3-MA (1 mM) for 24h. GAPDH was used as the loading control. (E) Western blot analysis for ATG5, Mcl1, and LC3I/II proteins in U266B1 wild-type and U266B1 ATG5 KD cells treated with AUTAC (5 μM) for 24h. GAPDH was used as the loading control. (F) The percentage of cellular viability for U266B1 cells treated with the indicated concentrations AUTAC for 24, 48 and 72h. (G) Viability of AC16 cardiomyocytes treated with the indicated concentrations of AUTAC for 24, 48 and 72h. Three biological replicates for each cell line were used to perform cell viability assay and Western blotting. * p < 0. 05, ** p < 0.01, and **** p < 0.0001 indicates statistical significance of each condition compared to the indicated control as determined using two-way ANOVA with Sidak’s post hoc test.
Rabbit Cst Bcl2 D17c4 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bcl-2+(d17c4)/Bax+Antibody/10__1142_slash_s0192415x23500295-238-59-60
Average 98 stars, based on 1 article reviews
rabbit cst bcl2 d17c4 rabbit mab - by Bioz Stars, 2026-09
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Image Search Results


(A) The chemical structure of Mcl1-specific AUTAC consists of a MCL1 binding moiety connected to a FBnG-tag via a pyrazole linker. (B) Western blot analysis for Mcl1, Bcl2, Bclxl, and LC3I/II proteins in U266B1 cells treated with the indicated concentrations of AUTAC for 24h and 48h. GAPDH was used as the loading control. (C) Confocal images with their quantification for Mcl1 and LC3B proteins in U266B1 cells treated with AUTAC (5 μM) for 24 and 48h (scale 10 μM). (D) Western blot analysis for Mcl1, and LC3I/II proteins in U266B1 cells treated with different combinations of AUTAC (5 μM), CQ (20 μM) or 3-MA (1 mM) for 24h. GAPDH was used as the loading control. (E) Western blot analysis for ATG5, Mcl1, and LC3I/II proteins in U266B1 wild-type and U266B1 ATG5 KD cells treated with AUTAC (5 μM) for 24h. GAPDH was used as the loading control. (F) The percentage of cellular viability for U266B1 cells treated with the indicated concentrations AUTAC for 24, 48 and 72h. (G) Viability of AC16 cardiomyocytes treated with the indicated concentrations of AUTAC for 24, 48 and 72h. Three biological replicates for each cell line were used to perform cell viability assay and Western blotting. * p < 0. 05, ** p < 0.01, and **** p < 0.0001 indicates statistical significance of each condition compared to the indicated control as determined using two-way ANOVA with Sidak’s post hoc test.

Journal: bioRxiv

Article Title: Proteasome Inhibition Enhances Lysosome-mediated Targeted Protein Degradation

doi: 10.1101/2025.01.31.634950

Figure Lengend Snippet: (A) The chemical structure of Mcl1-specific AUTAC consists of a MCL1 binding moiety connected to a FBnG-tag via a pyrazole linker. (B) Western blot analysis for Mcl1, Bcl2, Bclxl, and LC3I/II proteins in U266B1 cells treated with the indicated concentrations of AUTAC for 24h and 48h. GAPDH was used as the loading control. (C) Confocal images with their quantification for Mcl1 and LC3B proteins in U266B1 cells treated with AUTAC (5 μM) for 24 and 48h (scale 10 μM). (D) Western blot analysis for Mcl1, and LC3I/II proteins in U266B1 cells treated with different combinations of AUTAC (5 μM), CQ (20 μM) or 3-MA (1 mM) for 24h. GAPDH was used as the loading control. (E) Western blot analysis for ATG5, Mcl1, and LC3I/II proteins in U266B1 wild-type and U266B1 ATG5 KD cells treated with AUTAC (5 μM) for 24h. GAPDH was used as the loading control. (F) The percentage of cellular viability for U266B1 cells treated with the indicated concentrations AUTAC for 24, 48 and 72h. (G) Viability of AC16 cardiomyocytes treated with the indicated concentrations of AUTAC for 24, 48 and 72h. Three biological replicates for each cell line were used to perform cell viability assay and Western blotting. * p < 0. 05, ** p < 0.01, and **** p < 0.0001 indicates statistical significance of each condition compared to the indicated control as determined using two-way ANOVA with Sidak’s post hoc test.

Article Snippet: The antibodies used were LC3B (Cat No:2775S; Cell Signaling) at 1:1,000, NRF1 (Cat No:8052S; Cell Signaling) at 1:1,000, cleaved caspase-3 (Cat No:9661L; Cell Signaling) at 1:500, ATG5 (Cat No: 12994S; Cell Signaling) at 1:1000, SQSTM1/p62 (Cat No:5114S; Cell Signaling) at 1:1000, Mcl1 (Cat No: 5453S; Cell Signaling) at 1:500, Bcl2 (Cat No: 3498S; Cell Signaling) at 1:1,000, Bclxl (Cat No: 2764 S; Cell Signaling) at 1:1,000, and GAPDH (Cat No: 5174S; Cell Signaling) at 1:10,000.

Techniques: Binding Assay, Western Blot, Control, Viability Assay